Research topic: Influenza D and porcine epidemic diarrhoea pseudotyped lentiviruses, production, neutralisation and serological surveillance.
Influenza D virus (IDV), member of Orthomyxoviridae, can infect swine, cattle, camelid, equine, and small ruminants, causing respiratory diseases. Porcine epidemic diarrhoea virus (PEDV), member of Coronaviridae, is a causative agent for the majority of lethal watery diarrhoea in neonatal pigs. Pseudotyped lentivirus (PV), containing reporter genes and interested glycoproteins on the viral particles, open the opportunities to study the glycoproteins of the pathogenic viruses at biosafety level 1. My project is to study the pseudotyped lentivirus of IDV and PEDV in several aspects; optimising production, validating neutralisation with vaccinated pig serum, and serological surveying with serum from the farm animals (pigs, cows, and sheep). This platform technology can be applied to the other strains of these viruses.
Supervisors: Dr Nigel Temperton and Dr Simon Scott Research Group: Biological Sciences